FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438316898
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCTC29628140.6759524931662572Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG5087750.092.129899
GAGCGTC5506550.085.092568
AGAGCGT5783650.081.10677
ATCGGAA5901700.081.017211
TCGGAAG6037500.078.5442352
CGGAAGA6203100.076.2716143
AAGAGCG6250000.075.393496
GAAGAGC9093750.052.1694455
GGAAGAG12622200.037.9500244
CGCCGTA3685900.025.28054655-59
TCGCCGT3828900.024.28601555-59
GCCGTAT3896850.023.9559355-59
GTCGCCG3996150.023.31964955-59
TGGTCGC4105200.022.86386750-54
CCGTATC4030750.022.7982355-59
CGTATCA4098200.022.56051660-64
GGTCGCC4171400.022.26575550-54
GTGGTCG4251850.022.15061850-54
TCTCGGT4637700.020.49223945-49
TCTTCGC4708950.020.14444730-34
Produced by FastQC (version 0.11.5)