FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407637491
Sequences flagged as poor quality0
Sequence length151
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC4162170.10210469085631772Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1280200.049.2856149
GAGCGTC1612450.039.2477768
ATCGGAA1784550.036.2265741
TCGGAAG1906850.033.782962
AGAGCGT1982200.032.179067
CGGAAGA2144650.030.0602343
AAGAGCG2355500.027.3748636
CGCCGTA732850.017.4416255-59
TCGCCGT854850.015.03045555-59
GAAGAGC4887950.013.7988675
GTCGCCG939750.013.6910755-59
GCCGTAT965300.013.5329655-59
GTACGTC1062200.012.25047330-34
CCGTATC1066450.011.98836355-59
TGGTCGC1102750.011.87130750-54
GGTCGCC1098550.011.6276250-54
TACGTCC1146350.011.5017130-34
CGTATCA1149800.011.47457360-64
GTGGTCG1216700.010.88940650-54
GCGTCGT1234050.010.481606510-14
Produced by FastQC (version 0.11.5)