FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291210680
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG6525060.224066644808494TruSeq Adapter, Index 19 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2774550.039.193761
TCGGAAG2790450.038.914282
CGGAAGA2939000.036.9219783
AGAGCAC4253350.025.8022467
AGCACAC4405800.024.9142959
GAGCACA4521800.024.2719528
GAAGAGC4816050.022.9154765
AAGAGCA6314150.017.6874586
GGAAGAG6970400.016.1219884
TATGCCG1444100.015.35522145-49
CTCGTAT1438750.014.47817140-44
CGTATGC1526200.014.37342845-49
TCTCGTA1481750.014.10595840-44
TCGTATG1544850.013.32897740-44
ATGCCGT1684250.013.30522545-49
TATCTCG1557850.013.22612540-44
GCCGTCT1701800.012.94730450-54
TGCCGTC1708300.012.88708545-49
ATCTCGT1659000.012.66876340-44
CGTCTTC1833500.012.30589450-54
Produced by FastQC (version 0.11.5)