FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347560649
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG10826040.3114863558676345TruSeq Adapter, Index 3 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA5190400.038.1602441
TCGGAAG5212950.037.9657062
CGGAAGA5280600.037.471153
AGAGCAC6872500.028.9639877
AGCACAC6962350.028.559989
GAGCACA7146950.027.849718
GAAGAGC7466500.026.771675
AAGAGCA9273300.021.7209746
GGAAGAG10232450.019.8236524
TATGCCG2288600.017.5716845-49
CGTATGC2437300.016.319445-49
ATGCCGT2492200.016.100145-49
GCCGTCT2479200.015.80490250-54
TGCCGTC2496400.015.78264245-49
CTCGTAT2285250.015.57122740-44
CGTCTTC2628650.015.19422650-54
TCTCGTA2322950.015.15375440-44
GCGCATC2613650.015.10732335-39
CCGTCTT2694100.014.79711750-54
TCGTATG2384850.014.62973240-44
Produced by FastQC (version 0.11.5)