FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347560649
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTC12453470.3583107016237618Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG4352750.045.5889639
GAGCGTC4712700.042.114628
AGAGCGT5008650.039.6753357
ATCGGAA5427850.036.88451
TCGGAAG5477450.036.518132
AAGAGCG5491450.036.3414736
CGGAAGA5575650.035.8586653
GAAGAGC7725100.026.1401165
CGCCGTA2021450.019.6592755-59
GGAAGAG10542700.019.451884
TCGCCGT2136400.018.5716555-59
GCCGTAT2195350.018.20232655-59
GTCGCCG2266450.017.49385355-59
CGTATCA2312900.017.18750460-64
CCGTATC2289200.017.17061655-59
TGGTCGC2389300.016.72646950-54
GGTCGCC2431550.016.19509550-54
GTGGTCG2531450.015.89196150-54
TCTCGGT2931600.013.92870245-49
TCGGTGG2935500.013.63329545-49
Produced by FastQC (version 0.11.5)