FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449228636
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1322050.039.2065969
GAGCGTC1708550.030.5156148
ATCGGAA1821800.028.9961471
TCGGAAG1977350.026.768062
AGAGCGT2078500.025.3189747
CGGAAGA2207300.024.0279053
AAGAGCG2475550.021.4259996
CGCCGTA739600.014.33490455-59
TCGCCGT894100.011.93241755-59
GCCGTAT984450.011.02135255-59
GAAGAGC5270150.010.6530215
GTCGCCG1014900.010.59212855-59
CCGTATC1118600.09.65683455-59
GTACGTC1114300.09.612287530-34
TGGTCGC1157200.09.42160150-54
CGTATCA1192900.09.2035960-64
GGTCGCC1193200.09.0377250-54
TACGTCC1221900.08.93073330-34
GTGGTCG1286500.08.58060150-54
GCGTCGT1270250.08.36929710-14
Produced by FastQC (version 0.11.5)