FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450799408
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATG6489090.1439462848629118TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1731900.067.46611
TCGGAAG1902650.061.6875762
CGGAAGA2144700.054.7355773
AGAGCAC4716400.025.3507277
AGCACAC4982850.023.9238519
GAGCACA5136850.023.35628
GAAGAGC5231000.022.9839655
TATGCCG1234150.019.26579545-49
CGTATGC1365050.017.26112245-49
CTCGTAT1234050.017.09958840-44
TCTCGTA1282200.016.44614840-44
GTCACGA1492700.016.0854825-29
AAGAGCA7700800.015.8959286
ATGCCGT1524750.015.66812345-49
TGCCGTC1552350.015.14858145-49
GCCGTCT1581600.014.94417650-54
TCGTATG1413100.014.7718740-44
ACGAGAT1651600.014.66459230-34
ATCTCGT1520000.014.09542840-44
GGAAGAG8831400.014.0101934
Produced by FastQC (version 0.11.5)