FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450799408
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCTC7583190.1682165030704743Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2104600.056.0494589
GAGCGTC2470450.047.881748
ATCGGAA2704200.044.485911
TCGGAAG2880200.041.7567942
AGAGCGT2841900.041.6643687
CGGAAGA3093600.038.8114553
AAGAGCG3325150.035.9384236
GAAGAGC6180250.019.8742395
CGCCGTA1469600.016.34738755-59
TCGCCGT1621050.014.89969555-59
GCCGTAT1726050.014.08486355-59
GTCGCCG1761050.013.69626455-59
CCGTATC1843500.012.98225555-59
TGGTCGC1882500.012.94564450-54
CGTATCA1912950.012.70965160-64
GGAAGAG10030750.012.6766634
GGTCGCC1922400.012.50273950-54
GCGTCGT1983400.012.075273510-14
GTGGTCG2042250.012.03949150-54
CGTCGTG2051600.011.70919710-14
Produced by FastQC (version 0.11.5)