FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430209839
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG6211450.14438186756579502TruSeq Adapter, Index 7 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1776800.061.795491
TCGGAAG1906950.057.516542
CGGAAGA2124000.051.6849823
AGAGCAC4552750.024.5951567
AGCACAC4849250.023.0659359
GAGCACA4944450.022.711138
GAAGAGC5037850.022.3331765
TATGCCG1214850.019.48314345-49
CGTATGC1339950.017.53127745-49
TCGTATC1363750.017.36914435-39
CTCGTAT1235250.017.08937340-44
TCTCGTA1274500.016.55285840-44
GTCACGA1465200.016.25933825-29
ATGCCGT1504800.015.81757145-49
TATCTCG1335150.015.63612840-44
AAGAGCA7422600.015.4689786
GCCGTCT1538350.015.23915350-54
ATTCGTA1568150.015.07839835-39
TGCCGTC1519750.014.6084445-49
GAATTCG1650300.014.54889430-34
Produced by FastQC (version 0.11.5)