FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430209839
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC6845300.15911537532269224Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1825550.059.9865769
GAGCGTC2174250.050.445178
ATCGGAA2380400.046.8496861
TCGGAAG2519000.044.1072732
AGAGCGT2506550.044.000217
CGGAAGA2718550.040.929433
AAGAGCG2931650.037.7796
GAAGAGC5621250.020.2694325
CGCCGTA1272500.018.3788555-59
TCGCCGT1416400.016.54803355-59
GCCGTAT1515550.015.58353155-59
GTCGCCG1550150.015.12485555-59
TGGTCGC1692150.014.08974250-54
CGTATCA1720750.013.81450160-64
CCGTATC1632350.013.61145855-59
GCGTCGT1730700.013.55998410-14
GTGGTCG1819200.013.21775250-54
CGTCGTG1781550.013.18419610-14
GGTCGCC1717700.013.00210650-54
GGAAGAG9062300.012.912274
Produced by FastQC (version 0.11.5)