FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456561401
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCTC51944401.137730870069763Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG8115100.099.639849
GAGCGTC8553300.094.725818
AGAGCGT8684500.093.392417
ATCGGAA8792200.093.324831
TCGGAAG8886600.091.951432
CGGAAGA9227050.088.520233
AAGAGCG9204500.088.366776
GAAGAGC12121750.067.3905945
GGAAGAG15433550.053.288764
CGCCGTA6704100.024.36015955-59
GCCGTAT6769000.024.06063555-59
CGTATCA6862000.023.459260-64
CCGTATC6892750.023.27825555-59
TGGTCGC7217500.022.78532250-54
TCGCCGT7319900.022.2993355-59
GTGGTCG7462550.022.07674450-54
TCTCGGT7540150.021.84757645-49
GGTCGCC7705050.021.09574550-54
TTCGCCT7765800.021.00377330-34
GTCTTCG7748100.020.85657530-34
Produced by FastQC (version 0.11.5)