FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454227723
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG12723100.28010399532571023TruSeq Adapter, Index 13 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA4822600.052.0923961
TCGGAAG4894300.051.0275152
CGGAAGA5034000.049.566433
AGAGCAC7357450.034.1486937
AGCACAC7606250.033.0443159
GAGCACA7764450.032.3801428
GAAGAGC8074300.031.208495
AAGAGCA10485650.024.2790836
GGAAGAG11579600.022.0781424
TATGCCG2679700.018.85454845-49
CGTATGC2830350.017.62713645-49
ATGCCGT2975750.016.9378345-49
CTCGTAT2461350.016.675340-44
GCCGTCT2978750.016.56886350-54
TGCCGTC3007350.016.4889245-49
TCTCGTA2528050.016.1166340-44
CGTCTTC3237750.015.54256750-54
TCGTATG2603600.015.53258540-44
CCGTCTT3277100.015.33204950-54
ATCTCGT2796500.014.63224540-44
Produced by FastQC (version 0.11.5)