FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454227723
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCTC15890160.34982805309749887Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG4442750.056.1109249
GAGCGTC4901150.050.927978
AGAGCGT5178550.048.293547
ATCGGAA5416550.047.2957761
TCGGAAG5498000.046.1181952
CGGAAGA5642800.044.7979773
AAGAGCG5703450.044.2137156
GAAGAGC8655150.029.5045015
GGAAGAG12243250.021.1698634
CGCCGTA2582350.019.4385355-59
GCCGTAT2792400.018.07651755-59
TCGCCGT2786250.018.01236255-59
CCGTATC2911700.017.04313555-59
CGTATCA2949000.016.97048260-64
GTCGCCG2992200.016.84235255-59
TGGTCGC3024950.016.6703950-54
GTGGTCG3159600.015.95950350-54
GGTCGCC3132900.015.94745350-54
TCTCGGT3629950.014.14009845-49
TCGGTGG3623600.013.90404645-49
Produced by FastQC (version 0.11.5)