FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435959955
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCTC11671640.26772275449014576Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2694950.068.3509149
GAGCGTC3045650.060.570868
ATCGGAA3240050.057.8217241
TCGGAAG3395600.054.9899832
AGAGCGT3397600.054.3540087
CGGAAGA3590200.051.91173
AAGAGCG3842100.048.4243856
GAAGAGC6614700.028.5664485
GGAAGAG10187550.018.8934274
CGCCGTA1975350.018.83175755-59
TCGCCGT2133300.017.44288655-59
GCCGTAT2208100.016.95180355-59
GTCGCCG2282750.016.30472455-59
CCGTATC2319800.015.97119955-59
CGTATCA2385100.015.60887960-64
TGGTCGC2442850.015.41603250-54
GGTCGCC2460600.015.17048550-54
GTGGTCG2588850.014.60207750-54
GCGTCGT2610750.014.2457310-14
CGTCGTG2668650.013.9382810-14
Produced by FastQC (version 0.11.5)