FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356532525
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATG13383480.375378936325655TruSeq Adapter, Index 8 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT6762910.18968563947987635TruSeq Adapter, Index 8 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA4783200.053.5178031
TCGGAAG4860450.052.5923652
CGGAAGA5021150.050.864493
GATCGGA2900950.037.8418921
AGAGCAC6837750.037.5380677
AGCACAC7020600.036.5149659
GAGCACA7171850.035.777238
GAAGAGC7325250.035.146765
AAGAGCA9270800.027.9281046
GGAAGAG10162550.025.6377944
TATGCCG3243450.022.35561445-49
CGCGCAT3276800.021.86459435-39
TCGCGCA3354300.021.44197735-39
TCTCGCG3411100.021.2862530-34
CGTATGC3382350.021.27681245-49
GCGCATC3417750.021.04136535-39
CTCGTAT3027800.020.9252840-44
ATGCCGT3439800.020.84474645-49
TCTCGTA3063300.020.58386440-44
GCCGTCT3435950.020.55251350-54
Produced by FastQC (version 0.11.5)