FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356532525
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC16019900.44932506508347303Illumina Single End PCR Primer 1 (96% over 33bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT7147570.20047455698466782Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG4428400.057.5596169
GAGCGTC4748800.053.6959348
AGAGCGT5057500.050.5332957
ATCGGAA5167100.050.2388461
TCGGAAG5229750.049.4577832
CGGAAGA5395900.047.8812983
AAGAGCG5463150.047.2075466
GATCGGA3246750.034.0721931
GAAGAGC7717550.033.719365
GGAAGAG10693900.024.6455524
CGCCGTA3102800.023.21273855-59
TCGCCGT3226850.022.2709455-59
GCCGTAT3294400.021.88030855-59
GTCGCCG3369750.021.53089355-59
CGTATCA3436600.020.86351660-64
TGGTCGC3492050.020.8460750-54
GTGGTCG3647550.020.04124650-54
TCTCGGT3998200.018.50012245-49
CTCGGTG4131700.017.88446445-49
CGGTGGT4109350.017.76929350-54
Produced by FastQC (version 0.11.5)