FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100864932
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATG8413520.8341372797435683TruSeq Adapter, Index 8 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT3274400.3246321526296176TruSeq Adapter, Index 8 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2138850.066.4655761
TCGGAAG2167350.065.59792
CGGAAGA2208300.064.3497243
AGAGCAC2712300.052.4689067
AGCACAC2750700.051.6075749
GAGCACA2794600.050.9189578
GAAGAGC2859850.049.8733675
GATCGGA1134000.047.2164881
AAGAGCA3378900.042.3043026
GGAAGAG3654750.039.2693144
TATGCCG1623400.024.12083845-49
CGCGCAT1633000.023.57841535-39
CTCGTAT1567250.023.53168940-44
TCTCGTA1578050.023.30081640-44
CGTATGC1665100.023.29473745-49
ATGCCGT1677300.023.29471845-49
TCGCGCA1651500.023.19665535-39
TCTCGCG1674350.023.14849530-34
GCGCATC1674250.023.0754335-39
GCCGTCT1685800.022.87952250-54
Produced by FastQC (version 0.11.5)