FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438231624
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG17349910.3959073022078388TruSeq Adapter, Index 1 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA3086550.0102.346391
TCGGAAG3255350.096.981282
CGGAAGA3481650.090.504663
AGAGCAC5930350.053.2696087
AGCACAC6246450.050.3278969
GAGCACA6307500.050.0212068
GAAGAGC6454800.049.0986675
AAGAGCA8854900.036.0165256
GGAAGAG9851550.032.591914
TATGCCG2583600.024.45769745-49
CGTATGC2712550.023.10742445-49
AGCGATA2750700.023.05511330-34
CTCGTAT2445050.023.00582540-44
CGATAGA2735550.022.8877635-39
GCGATAG2747100.022.74967830-34
TCTCGTA2470000.022.70065340-44
ATGCCGT2855550.022.08631545-49
TGCCGTC2876150.021.60149445-49
CAGCGAT2967800.021.355430-34
GCCGTCT2906000.021.33878950-54
Produced by FastQC (version 0.11.5)