FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455350004
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC6963990.15293708002251385Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1924550.055.9812559
GAGCGTC2285150.047.2234658
ATCGGAA2436650.045.1234171
TCGGAAG2594000.042.2623182
AGAGCGT2630650.041.181147
CGGAAGA2862300.038.2977333
AAGAGCG3085650.035.4406366
GAAGAGC5975800.018.8157715
CGCCGTA1304500.016.8522655-59
TCGCCGT1457900.015.10890155-59
GCCGTAT1532750.014.46566755-59
GTCGCCG1620950.013.67229355-59
CCGTATC1661350.013.13124455-59
CGTATCA1726500.012.86129760-64
TGGTCGC1767950.012.6699450-54
GGTCGCC1819700.012.11998750-54
GGAAGAG9610550.012.0994754
GCGTCGT1831000.012.02406310-14
GTGGTCG1896900.011.92406550-54
CGTCGTG1892000.011.68007510-14
Produced by FastQC (version 0.11.5)