FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460196106
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT94255972.0481696557423716TruSeq Adapter, Index 27 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG15424070.33516298375632064TruSeq Adapter, Index 27 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA11246250.0135.131761
ATCGGAA13404350.0113.467952
TCGGAAG13576250.0111.924813
CGGAAGA13810100.0109.953844
AGAGCAC16353650.092.630168
GAGCACA16792100.090.171399
GAAGAGC16923200.089.6763156
AAGAGCA19374700.078.40587
GGAAGAG20566300.074.0731665
TATGCCG12803500.027.73889245-49
CTCGTAT12383350.027.4177940-44
CGTATGC12924000.027.41654645-49
TCTCGTA12431150.027.40694840-44
TACTCGA13010900.027.14668735-39
ATGCCGT13035900.027.01838945-49
ATCTCGT12662850.026.93374640-44
GCCGTCT13114650.026.88905550-54
ACTCGAT13297450.026.59080735-39
CGTCTGA13349200.026.55761515-19
ACACGTC13394000.026.49610110-14
Produced by FastQC (version 0.11.5)