FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences481288014
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG5625270.11687949494624231TruSeq Adapter, Index 3 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1726300.055.444711
TCGGAAG1891600.050.6540532
CGGAAGA2128750.045.0021323
AGAGCAC4925200.020.0536987
AGCACAC5223000.018.9001339
GAGCACA5363250.018.457178
TATGCCG1103950.018.2050745-49
GAAGAGC5475450.018.156235
GCGCATC1150950.017.2915835-39
TAATGCG1209050.016.62736530-34
CGTATGC1233600.016.24707845-49
CTCGTAT1155150.015.78399440-44
TCTCGTA1190050.015.26262740-44
AATGCGC1279850.015.14568130-34
TGCGCAT1330150.015.05685835-39
ATGCGCA1354500.014.772264535-39
ATGCCGT1412400.014.34325845-49
TGCCGTC1418850.013.74976845-49
CGCATCT1331100.013.72927835-39
GCCGTCT1476550.013.57225650-54
Produced by FastQC (version 0.11.5)