FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences481288014
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCTC6241720.12968783386323848Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1901950.050.5127689
GAGCGTC2309250.041.736568
ATCGGAA2487500.039.1493761
TCGGAAG2653600.036.7363742
AGAGCGT2694000.035.918887
CGGAAGA2893150.033.687763
AAGAGCG3135600.031.0816946
CGCCGTA1206700.016.61347855-59
GAAGAGC6199300.016.1950365
TCGCCGT1345800.014.9620455-59
GCCGTAT1469900.013.85564355-59
GTCGCCG1502700.013.41525955-59
TGGTCGC1645000.012.43652450-54
CCGTATC1600400.012.29465855-59
CGTATCA1673300.012.16083660-64
GGTCGCC1683700.011.6917850-54
GTGGTCG1779300.011.57359450-54
GCGTCGT1841250.010.87967510-14
CAGTACG1798950.010.80904130-34
CGTCGTG1899250.010.57339810-14
Produced by FastQC (version 0.11.5)