FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455559177
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1098900.024.7887731
TCGGAAG1249800.021.894882
CGGAAGA1449700.018.8707793
TATGCCG632850.09.98544445-49
CGGCTAT708300.08.9590230-34
CGTATGC772800.08.33099445-49
CTCGTAT710300.08.04366640-44
TCTCGTA744500.07.711173540-44
AGAGCAC4104150.07.37534147
ATGCCGT915550.07.17300645-49
ACCGGCT914400.07.16649130-34
AGCACAC4424700.06.893659
GCCGTCT955300.06.828667650-54
GAGCACA4566850.06.6965478
GAAGAGC4639650.06.59595875
GTCACCG1037950.06.480617525-29
TGCCGTC922450.06.29565145-49
TCACCGG954400.06.19714625-29
ATCTCGT977200.06.09008840-44
CACCGGC1124350.06.00240430-34
Produced by FastQC (version 0.11.5)