FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483287668
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1779850.046.6123661
TCGGAAG1942950.042.802082
CGGAAGA2168850.038.390813
AGAGCAC4875300.017.5776547
TATGCCG1024850.016.7724445-49
AGCACAC5193700.016.4958699
GAGCACA5318100.016.15778
GAAGAGC5462050.015.7622145
CGTATGC1172900.014.64297145-49
CTCGTAT1058700.014.25934940-44
TCTCGTA1101300.013.68144940-44
ATGCCGT1322350.013.15467145-49
TGCCGTC1348350.012.54193445-49
GCCGTCT1397250.012.27431950-54
TCGTATG1243400.011.92318840-44
ATCTCGT1356000.011.26664940-44
ACTCCGG1557800.011.22743130-34
CCGGAGA1541400.011.0825930-34
AAGAGCA8096500.010.987196
CACTCCG1591650.010.90121230-34
Produced by FastQC (version 0.11.5)