FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441487885
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATG5780610.13093473674821224TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2664750.038.7063831
TCGGAAG2709300.037.8887632
CGGAAGA2927000.035.134633
AGAGCAC5181500.020.2151227
AGCACAC5363400.019.498659
GAGCACA5521300.018.9944888
GAAGAGC5877500.018.014865
TATGCCG1373750.015.14659645-49
CGTATGC1520150.013.62535445-49
AAGAGCA8220800.013.1338136
CTCGTAT1461600.012.88340540-44
TCTCGTA1507900.012.4820940-44
ATGCCGT1716300.012.21669245-49
GGAAGAG9139650.011.9215314
TGCCGTC1728300.011.91353145-49
GCCGTCT1733500.011.8849350-54
TCGTATG1591900.011.67325640-44
GTCACGA1863100.011.35413325-29
ACGAGAT1970600.010.81919830-34
ATCTCGT1765100.010.76527140-44
Produced by FastQC (version 0.11.5)