FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441487885
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTC6611480.1497545057210347Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2407900.042.7432679
GAGCGTC2802500.036.8539738
ATCGGAA3161200.033.206871
AGAGCGT3140150.033.0279587
TCGGAAG3218950.032.476182
CGGAAGA3441450.030.4481413
AAGAGCG3553100.029.4606556
GAAGAGC6424950.016.7828065
CGCCGTA1359050.015.37591855-59
TCGCCGT1552500.013.49546155-59
GCCGTAT1622700.013.04379355-59
GTCGCCG1632100.012.86390455-59
CCGTATC1757150.011.87338255-59
TGGTCGC1811300.011.83017550-54
CGTATCA1825300.011.5987160-64
GGTCGCC1818550.011.54158350-54
GGAAGAG9785850.011.3971654
GTGGTCG1907000.011.20534350-54
CCGTGTA2198500.09.81860835-39
TCTCGGT2293900.09.53313845-49
Produced by FastQC (version 0.11.5)