FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences469085003
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG6228930.13278893932151567TruSeq Adapter, Index 1 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1861100.060.116951
TCGGAAG2028900.054.874222
CGGAAGA2256850.049.337863
AGAGCAC4941300.022.9193367
AGCACAC5293400.021.4782529
GAGCACA5406350.021.0268488
GAAGAGC5530800.020.7081995
TATGCCG1222800.018.79285645-49
CGTATGC1350800.016.88127145-49
CGATAGA1377550.016.57632435-39
AGCGATA1394400.016.55977630-34
CTCGTAT1246700.016.50129340-44
TCTCGTA1286750.016.01920740-44
GCGATAG1398600.015.94666230-34
ATGCCGT1509700.015.29632545-49
TGCCGTC1536250.014.50218245-49
GCCGTCT1569900.014.485188550-54
AAGAGCA8077600.014.4551636
TCGTATG1410300.014.150619540-44
CAGCGAT1667950.014.00456130-34
Produced by FastQC (version 0.11.5)