FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466383712
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG8950190.19190614444099624TruSeq Adapter, Index 13 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA5024250.031.8721891
TCGGAAG5047100.031.6100542
CGGAAGA5213800.030.646193
AGAGCAC7414750.021.8275957
AGCACAC7586350.021.4007459
GAGCACA7805750.020.793658
GAAGAGC8247500.019.710295
TATGCCG2276600.015.72742545-49
AAGAGCA10649650.015.51176556
CGTATGC2437150.014.57060645-49
GGAAGAG11825300.014.0426284
CTCGTAT2305050.013.99050640-44
ATGCCGT2638800.013.62857345-49
TCTCGTA2368700.013.5870240-44
GCCGTCT2684150.013.12572550-54
CGTCTTC2892100.012.44062850-54
ATCTCGT2657850.012.20214840-44
CCGTCTT2953150.012.18000850-54
TGCCGTC2654100.012.08335745-49
TCGTATG2441450.011.75112140-44
Produced by FastQC (version 0.11.5)