FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466383712
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTC10268000.22016206260650886Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG4588900.034.8599439
GAGCGTC5050100.031.7124948
AGAGCGT5439100.029.5374687
ATCGGAA5785000.028.1884921
TCGGAAG5838400.027.7791672
CGGAAGA5989450.027.0767123
AAGAGCG6004700.026.9590286
GAAGAGC9047900.018.280125
CGCCGTA2252100.015.83370355-59
TCGCCGT2451250.014.52778855-59
GCCGTAT2498250.014.38620255-59
GTCGCCG2599950.013.77495855-59
CGTATCA2647250.013.51988960-64
GGAAGAG12653350.013.3306734
TGGTCGC2760600.013.10189850-54
GTGGTCG2913650.012.48134250-54
CCGTATC2608650.012.354671555-59
GGTCGCC2793850.011.64250150-54
TCTCGGT3442150.010.70639645-49
CCGTGTA3513050.010.42974135-39
Produced by FastQC (version 0.11.5)