FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477400013
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCTC5001890.10477356229146144Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1790250.043.722069
GAGCGTC2189350.035.8299758
ATCGGAA2443700.033.0242651
AGAGCGT2554050.030.8886267
TCGGAAG2617200.030.6772542
CGGAAGA2828200.028.2838423
AAGAGCG2998800.026.582736
CGCCGTA1036400.015.46429555-59
TCGCCGT1176650.013.630897555-59
GAAGAGC6102650.013.6078585
GCCGTAT1288800.012.67537655-59
GTCGCCG1315500.012.26821755-59
TGGTCGC1448250.011.31369950-54
CCGTATC1416900.011.28484655-59
CGTATCA1496600.010.97100560-64
GGTCGCC1500750.010.6918750-54
GTGGTCG1580200.010.47448850-54
CAGTACG1595800.010.02927430-34
AGTACGT1714200.09.58899335-39
GCGTCGT1720750.09.40089310-14
Produced by FastQC (version 0.11.5)