FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436833207
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1317450.032.2436331
TCGGAAG1441750.029.5061572
CGGAAGA1641200.025.9866923
CGCGAAA558700.015.39677835-39
TCCGCGA584100.014.848598530-34
CCGCGAA605450.013.93226530-34
TATGCCG701450.012.72624845-49
AGAGCAC4115900.010.9325357
CGTATGC840700.010.61832545-49
CTCCGCG848000.010.49610230-34
AGCACAC4442300.010.260729
GCGAAAT873300.010.18556935-39
GAGCACA4546800.09.9706658
CTCGTAT757000.09.83922840-44
GAAGAGC4629650.09.8222915
ATGCCGT964150.09.51740345-49
TCTCGTA792300.09.38255240-44
ACTCCGC972950.09.18987730-34
TGCCGTC967450.09.15673445-49
GCCGTCT1003000.08.95930950-54
Produced by FastQC (version 0.11.5)