FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467925697
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATG5199040.1111082386227658TruSeq Adapter, Index 8 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1731300.056.106251
TCGGAAG1889450.051.595942
CGGAAGA2132200.045.752343
AGAGCAC4761950.021.0219547
CGCGCAT951100.020.46197735-39
TCGCGCA988550.019.86007135-39
AGCACAC5095900.019.6841139
GAGCACA5175450.019.4151388
GAAGAGC5319000.018.9782945
TCTCGCG1049900.018.87623230-34
TATGCCG1082350.018.53282745-49
GCGCATC1139700.017.31023835-39
CGTATGC1204050.016.36420845-49
CTCGTAT1100100.016.04475840-44
TCTCGTA1143950.015.34907240-44
CTCGCGC1277300.015.20265230-34
ATGCCGT1367300.014.70343945-49
ACTCTCG1415350.014.27557230-34
TGCCGTC1399450.014.0181345-49
GCCGTCT1437400.013.79800750-54
Produced by FastQC (version 0.11.5)