FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458886177
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG6566370.14309365435516266TruSeq Adapter, Index 19 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG6027000.019.910372
ATCGGAA6042950.019.8201941
CGGAAGA6108900.019.661233
AGAGCAC8199050.014.9017837
AGCACAC8334800.014.6886439
TATGCCG2355100.014.62185745-49
GAGCACA8556150.014.3484588
GAAGAGC9019750.013.6705395
CGTATGC2543750.013.44912445-49
ATGCCGT2645700.013.03992445-49
GCCGTCT2665300.012.78521450-54
CTCGTAT2448700.012.76238440-44
TCTCGTA2540800.012.32658740-44
CGTCTTC2888050.012.01446550-54
CCGTCTT2977700.011.66150850-54
TATCTCG2698550.011.49909140-44
ATCTCGT2820800.011.14516440-44
AAGAGCA11443350.010.9715916
GATCGGA5101150.010.3114551
GGAAGAG12620300.010.029954
Produced by FastQC (version 0.11.5)