FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458886177
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC7390040.16104298561165856Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG5020150.023.7068849
GAGCGTC5506400.021.6644428
AGAGCGT5920750.020.2387737
TCGGAAG6423500.018.8798142
ATCGGAA6450850.018.8246291
AAGAGCG6488900.018.651486
CGGAAGA6526050.018.5883733
CGCCGTA2078550.016.37629555-59
TCGCCGT2244200.015.17204355-59
GCCGTAT2296700.014.92308155-59
GTCGCCG2382850.014.40728155-59
CGTATCA2489250.013.7165960-64
TGGTCGC2610500.013.27994250-54
GAAGAGC9435000.013.1727275
GTGGTCG2789950.012.50061550-54
TCTCGGT3287650.010.73503445-49
CGGTGGT3422700.010.26166450-54
CTCGGTG3447300.010.254745-49
CCGTATC2423900.09.86291655-59
GGAAGAG13124450.09.7493414
Produced by FastQC (version 0.11.5)