FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416247088
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC12086300.2903635928859639Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3980800.048.911349
GAGCGTC4378300.044.563258
AGAGCGT4739550.041.3339967
ATCGGAA5069850.039.3457371
TCGGAAG5099900.038.9124152
CGGAAGA5216350.037.990263
AAGAGCG5247450.037.637436
GAAGAGC7848600.025.570955
CGCCGTA2056450.018.84923255-59
GGAAGAG11159550.018.3468284
TCGCCGT2205700.017.51002755-59
GCCGTAT2264100.017.20822155-59
GTCGCCG2338550.016.71494155-59
CCGTATC2371650.016.23651355-59
CGTATCA2437050.015.93835960-64
TGGTCGC2497400.015.73404350-54
GGTCGCC2517500.015.48114350-54
GTGGTCG2660900.014.85770750-54
TCTCGGT3084950.013.07107745-49
TCGGTGG3130300.012.72793845-49
Produced by FastQC (version 0.11.5)