FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456653799
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC15026210.32905036666518567Illumina Single End PCR Primer 1 (96% over 33bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT5365090.11748703310360503Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG4456450.052.9911739
GAGCGTC4861500.048.619948
AGAGCGT5252100.045.137247
ATCGGAA5341100.044.920331
TCGGAAG5417600.044.2164152
CGGAAGA5595600.042.7466163
AAGAGCG5718500.041.7840966
GAAGAGC8617950.028.0990035
GATCGGA3459250.023.9962981
CGCCGTA2973150.021.28902855-59
GGAAGAG12242000.020.1037944
TCGCCGT3150550.020.0663655-59
GCCGTAT3233350.019.65071355-59
GTCGCCG3269450.019.43994555-59
TGGTCGC3447850.018.50695450-54
CGTATCA3430950.018.44265760-64
GTGGTCG3612700.017.72508850-54
TCTCGGT4047600.015.99503645-49
CTCGGTG4209300.015.37025345-49
CGGTGGT4218750.015.23067150-54
Produced by FastQC (version 0.11.5)