FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431894811
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATG6849740.15859741366515284TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA3472150.039.9546661
TCGGAAG3565750.038.7748222
CGGAAGA3714950.037.213313
AGAGCAC5969100.023.480787
AGCACAC6216550.022.641749
GAGCACA6382550.022.019938
GAAGAGC6555550.021.5128975
TATGCCG1710600.016.21885145-49
AAGAGCA8893450.016.09486
CGTATGC1873300.014.70650745-49
GGAAGAG10006850.014.4814934
ATGCCGT1974800.014.06221445-49
TGCCGTC1996200.013.81850245-49
GCCGTCT2025050.013.57313750-54
CTCGTAT1652250.013.46888340-44
TCTCGTA1719000.012.92985240-44
CGTCTTC2281500.012.31752750-54
TCGTATG1800950.012.29158440-44
GTCACGA2313400.012.11954525-29
CCGTCTT2329950.012.05454550-54
Produced by FastQC (version 0.11.5)