FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431894811
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCTC8719150.20188133262846725Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3041650.044.9591949
GAGCGTC3455600.039.621738
AGAGCGT3686350.037.338447
ATCGGAA3878100.036.0717281
TCGGAAG3976250.035.0588152
CGGAAGA4112600.033.965983
AAGAGCG4139000.033.4912226
GAAGAGC7007500.020.2006365
CGCCGTA1576900.017.46755655-59
TCGCCGT1752000.015.77061655-59
GCCGTAT1802500.015.44056655-59
GTCGCCG1894200.014.62190155-59
CCGTATC1937800.014.341536555-59
CGTATCA1973750.014.07635960-64
TGGTCGC1987250.014.07307750-54
GGAAGAG10458100.014.0064334
GGTCGCC2062150.013.48389250-54
GTGGTCG2105800.013.31316150-54
TCGGTGG2521550.011.25494645-49
TCTCGGT2558550.011.17944445-49
Produced by FastQC (version 0.11.5)