FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435611945
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATG5898570.13540882126177695TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1702350.065.569191
TCGGAAG1846400.060.3638762
CGGAAGA2065400.053.8930743
AGAGCAC4535850.025.018117
AGCACAC4870000.023.3505089
GAGCACA4943700.023.0229288
GAAGAGC5078200.022.4346795
CGCGAAA1046250.022.20125235-39
TCCGCGA1072800.021.8060130-34
CCGCGAA1102850.020.00252230-34
TATGCCG1214400.019.56797845-49
CGTATGC1321850.017.80733145-49
CTCCGCG1365000.017.3388130-34
GCGAAAT1369100.017.27303935-39
CTCGTAT1182850.017.20345940-44
TCTCGTA1219850.016.64123740-44
ATGCCGT1477250.016.09700445-49
ACTCCGC1492700.015.88461630-34
AAGAGCA7486200.015.5601536
GCCGTCT1519250.015.38053650-54
Produced by FastQC (version 0.11.5)