FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435611945
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC7292970.16741896276512802Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1811050.061.802429
GAGCGTC2140700.052.4061938
ATCGGAA2339200.048.843721
TCGGAAG2486650.045.799782
AGAGCGT2486900.045.2941637
CGGAAGA2730800.041.651523
AAGAGCG2942200.038.566186
GAAGAGC5663450.020.5154275
CGCCGTA1270750.018.68643255-59
TCGCCGT1403150.016.95316155-59
GCCGTAT1490650.016.0854455-59
GTCGCCG1542000.015.46515855-59
TGGTCGC1681550.014.36257650-54
CGTATCA1678500.014.26013460-64
CCGTATC1608750.014.03670455-59
GCGTCGT1730500.013.8075410-14
GTGGTCG1801500.013.49801950-54
CGTCGTG1775250.013.49214910-14
GGTCGCC1736050.013.15918750-54
GGAAGAG9181650.013.0220834
Produced by FastQC (version 0.11.5)