FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449076521
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG8024190.17868202020742027TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2038400.071.451171
TCGGAAG2202650.066.192082
CGGAAGA2390300.060.9922183
AGAGCAC4973200.029.6752957
AGCACAC5266800.028.1065169
GAGCACA5379450.027.5678048
GAAGAGC5505900.027.022755
TATGCCG1447650.020.39826845-49
AAGAGCA8047700.018.7418586
CGTATGC1597100.018.4350145-49
CTCGTAT1414050.018.28715340-44
TCTCGTA1467650.017.585740-44
ATGCCGT1734050.017.037645-49
GGAAGAG9051500.016.7996714
TGCCGTC1740500.016.60543345-49
GCCGTCT1781450.016.39221650-54
TCGTATG1575850.016.07185640-44
CCGGAGA1887100.015.5486830-34
ACTCCGG1926200.015.47844730-34
ATCTCGT1697750.015.34232740-44
Produced by FastQC (version 0.11.5)