FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449076521
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCTC9567150.21304052990113903Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2219400.066.274269
GAGCGTC2639600.055.8072058
ATCGGAA2767900.054.4391251
TCGGAAG2912050.051.4541662
AGAGCGT2931900.050.3942647
CGGAAGA3111100.048.1046873
AAGAGCG3340400.044.706686
GAAGAGC6161600.024.794635
CGCCGTA1536950.019.3555155-59
TCGCCGT1715550.017.35484355-59
GCCGTAT1802350.016.67028855-59
GTCGCCG1859350.016.11323555-59
GGAAGAG9774950.015.9801544
CCGTATC1912000.015.41548955-59
CGTATCA1989500.015.03069260-64
TGGTCGC2019800.015.00766150-54
GGTCGCC2044800.014.56890450-54
GTGGTCG2172000.014.01943850-54
GCGTCGT2210100.013.62639810-14
CGTCGTG2259250.013.37038810-14
Produced by FastQC (version 0.11.5)