FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467501619
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG6785540.14514473799073624TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1841050.065.676671
TCGGAAG1985000.060.9137572
CGGAAGA2246750.053.810753
AGAGCAC4876050.025.2032137
AGCACAC5191400.023.6806379
GAGCACA5330050.023.0755148
GAAGAGC5445950.022.6361145
TATGCCG1289800.019.21912445-49
CGGCTAT1357100.018.1889530-34
CTCGTAT1304150.017.22297340-44
CGTATGC1438200.016.91241645-49
TCTCGTA1346650.016.66326940-44
ACCGGCT1570400.015.82552230-34
ATGCCGT1582650.015.78652945-49
AAGAGCA8029650.015.696196
TGCCGTC1592550.015.28509945-49
TCACCGG1605600.015.14162825-29
GCCGTCT1626750.015.04263550-54
TCGTATG1485100.014.82671640-44
GTCACCG1691850.014.77075225-29
Produced by FastQC (version 0.11.5)