FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467501619
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC7704310.16479750415580915Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2133650.056.807659
GAGCGTC2524900.048.079138
ATCGGAA2669400.046.024051
TCGGAAG2821100.043.448222
AGAGCGT2908950.041.8736577
CGGAAGA3103450.039.5194323
AAGAGCG3350800.036.686556
GAAGAGC6308500.019.9643175
CGCCGTA1423800.017.25230855-59
TCGCCGT1592100.015.47683855-59
GCCGTAT1682250.014.77155355-59
GTCGCCG1736600.014.23494955-59
CCGTATC1805200.013.49000955-59
TGGTCGC1895050.013.21731350-54
CGTATCA1873800.013.1736760-64
GTACGTC1896250.012.99771630-34
GGAAGAG10054550.012.8743884
GGTCGCC1931300.012.78304150-54
TACGTCC1974150.012.62436930-34
GTGGTCG2041050.012.3194550-54
Produced by FastQC (version 0.11.5)