FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363115512
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG7211690.19860594663882056TruSeq Adapter, Index 13 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA3234850.039.0946921
TCGGAAG3312800.037.9943242
CGGAAGA3428750.036.7158053
AGAGCAC5279300.024.2019797
AGCACAC5454500.023.4631489
GAGCACA5593450.022.8906578
GAAGAGC5803350.022.127345
TATGCCG1481150.017.2283445-49
AAGAGCA7762400.016.8277236
CGTATGC1606800.015.67628545-49
CTCGTAT1497850.015.45824740-44
GGAAGAG8691500.015.128294
TCTCGTA1534900.015.04543840-44
ATGCCGT1709500.014.95501545-49
TGCCGTC1718100.014.60169245-49
GCCGTCT1737450.014.44647550-54
TCGTATG1613700.014.13014840-44
CGTCTTC1910000.013.45941550-54
ATCTCGT1733100.013.35995440-44
CCGTCTT1971250.013.04562350-54
Produced by FastQC (version 0.11.5)