FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363115512
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCTC8026310.2210401300619732Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3003300.042.0295079
GAGCGTC3329500.038.0532268
AGAGCGT3591500.035.3780987
ATCGGAA3851550.033.5282861
TCGGAAG3920250.032.736092
CGGAAGA4054450.031.6344493
AAGAGCG4056050.031.5726476
GAAGAGC6392550.020.448345
CGCCGTA1555150.016.312855-59
TCGCCGT1689700.015.03868755-59
GCCGTAT1710600.014.96166955-59
GGAAGAG9410400.014.2225274
CCGTATC1815550.013.92441355-59
CGTATCA1841150.013.86461160-64
GTCGCCG1861800.013.78084855-59
TGGTCGC1938250.013.35273550-54
GGTCGCC2012150.012.71470350-54
GTGGTCG2055150.012.63974650-54
TCTCGGT2471950.010.711534545-49
TCGGTGG2477250.010.51080645-49
Produced by FastQC (version 0.11.5)