FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288715047
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG9650930.3342718053763232TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2486450.064.063781
TCGGAAG2547100.062.5171172
CGGAAGA2689550.059.18973
AGAGCAC4148000.038.600367
AGCACAC4291250.037.2746249
GAGCACA4402900.036.37558
GAAGAGC4624950.034.8124585
AAGAGCA6135600.026.4480086
GGAAGAG6841200.023.8526084
TATGCCG1582650.020.29245-49
CTCGTAT1591650.019.1056940-44
CGTATGC1679200.018.94998645-49
TCTCGTA1627350.018.68744940-44
ATGCCGT1786650.017.99047345-49
TATCTCG1680150.017.88531940-44
TCGTATG1690950.017.86454440-44
TGCCGTC1824750.017.48374445-49
CGCTCAT1848250.017.33605430-34
GCCGTCT1857700.017.11069350-54
ATCTCGT1800900.016.97431840-44
Produced by FastQC (version 0.11.5)