FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288715047
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCTC9980450.34568513500441145Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2374750.066.5276649
GAGCGTC2627950.060.145388
ATCGGAA2888600.056.056381
AGAGCGT2832800.055.926617
TCGGAAG2956700.054.6064222
CGGAAGA3089700.052.2458723
AAGAGCG3174350.050.6854826
GAAGAGC5047950.032.2406435
GGAAGAG7343400.022.5073494
CGCCGTA1625600.019.37372655-59
GCCGTAT1763950.017.95448555-59
TCGCCGT1755050.017.83817955-59
CCGTATC1845050.017.02305455-59
GTCGCCG1880050.016.83416255-59
CGTATCA1870800.016.83248360-64
TGGTCGC1903950.016.6942550-54
GGTCGCC1967700.016.13496250-54
GTGGTCG1978100.016.08678450-54
TCTCGGT2273900.014.3685745-49
TCGGTGG2330200.013.93241645-49
Produced by FastQC (version 0.11.5)