FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251753675
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCTC11806130.46895561703319727Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2488950.074.594589
GAGCGTC2700350.068.784398
AGAGCGT2893850.064.30037
ATCGGAA3000300.062.722721
TCGGAAG3030050.061.84892
CGGAAGA3175750.058.9816633
AAGAGCG3208900.058.2505076
GAAGAGC4802500.039.3576745
GGAAGAG6793650.028.1669464
CGCCGTA1731600.021.01354655-59
GCCGTAT1839550.019.90100955-59
TCGCCGT1842650.019.72116755-59
CCGTATC1905400.018.90199755-59
CGTATCA1930000.018.76187160-64
GTCGCCG1945250.018.69441455-59
TGGTCGC1967000.018.54293650-54
GTGGTCG2050500.017.90169550-54
GGTCGCC2030500.017.88876750-54
TCTCGGT2273150.016.39800545-49
TCGGTGG2331900.015.77781145-49
Produced by FastQC (version 0.11.5)