FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004973639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004973639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393142238
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCTC14005760.35625172383538195Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT9474750.2410005612269013Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG4080100.054.470979
GAGCGTC4398550.050.5827948
GATCGGA2979400.050.4528661
ATCGGAA4677100.048.3698431
AGAGCGT4774800.046.7081157
TCGGAAG4837000.046.6925432
CGGAAGA5062300.044.5767173
AAGAGCG5283800.042.607086
GAAGAGC7737450.029.4650755
CGCCGTA3264000.022.67396255-59
TCGCCGT3371650.021.87391755-59
GCCGTAT3466600.021.3454755-59
GGAAGAG11110850.020.8810274
GTCGCCG3568750.020.79339655-59
CGTATCA3606200.020.38391360-64
TGGTCGC3701850.020.12263350-54
GTGGTCG3863950.019.3756350-54
GCGTCGT3942550.018.7515810-14
CGTCGTG3991650.018.52273610-14
TCTCGGT4209550.017.97487445-49
Produced by FastQC (version 0.11.5)